Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.242

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2487	0.2448
	B	25.00	0.2198	0.2134	0.2515
	C	8.33	0.1798	0.1969	0.2210
	D	2.78	0.1025	0.1342	0.1278
	E	0.93	0.04515	0.06875	0.05835
	F	0.31	0.02140	0.02630	0.02135
	G	0.10	0.008550	0.007550	0.009100
	H	0.03	0	0.0003000	0

Yield (mg/mL): 2.242     Dilution Factor:  100×

Download these data

Protein purified on 10/8/22 and assayed on 10/10/22.
Data uploaded by codliu and last updated on 2022-10-14 16:24:09.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	0.2487	0.2448
	B	31.3	0.2198	0.2134	0.2515
	C	33.6	0.1798	0.1969	0.2210
	D	37.7	0.1025	0.1342	0.1278
	E	42.4	0.04515	0.06875	0.05835
	F	45.7	0.02140	0.02630	0.02135
	G	48.3	0.008550	0.007550	0.009100
	H	50.0	0	0.0003000	0

Download these data

Protein purified on 10/8/22 and assayed on 10/9/22.
Data uploaded by codliu and last updated on 2022-10-14 16:35:58.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.