D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y422F

Expression Data

Yield (mg/mL): 2.83

Kinetic Assay Data

**slope of Y422F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1129	0.1121	0.1141
B	25.00	0.05735	0.0626	0.0549
C	8.33	0.0398	0.041	0.0459
D	2.78	0.01655	0.01685	0.0219
E	0.93	0.0052	0.00605
F	0.31	0.00205	0.002
G	0.10	0.00065	0.00075	0.00105
H	0.03	0	0.00045	0

Yield (mg/mL): 2.83 Dilution Factor: 100×

Protein purified on 8/30/2022 and assayed on 8/31/2022.
Data uploaded by mspiridonova and last updated on 2022-09-15 22:05:36.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2197	0.2196	0.2121
B	25.00	0.1718	0.1867	0.1728
C	8.33	0.1606	0.1489	0.1605
D	2.78	0.07765	0.071	0.0822
E	0.93	0.03135	0.0286	0.03775
F	0.31	0.01175	0.00955	0.0126
G	0.10	0.00485	0.00375	0.00415
H	0.03	0	0.00025	0

Yield (mg/mL): 2.804 Dilution Factor: 100×

Protein purified on 8/30/2022 and assayed on 8/31/2022.
Data uploaded by mspiridonova and last updated on 2022-09-15 21:48:35.

Design Data

Rosetta Score for Y422F: -0.362

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.