D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S169A

Expression Data

Yield (mg/mL): 1.346

Kinetic Assay Data

**slope of S169A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2002		0.1949
B	25.00	0.1699	0.1571	0.1682
C	8.33	0.1585	0.1595	0.1364
D	2.78	0.07535	0.07665	0.06935
E	0.93	0.0316	0.0328	0.0322
F	0.31	0.0107	0.01075	0.01015
G	0.10	0.00365	0.00365	0.0035
H	0.03	0.00015	0	0.00025

Yield (mg/mL): 1.346 Dilution Factor: 100×

Protein purified on 8/30/2022 and assayed on 8/31/2022.
Data uploaded by mspiridonova and last updated on 2022-09-15 22:00:23.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2197	0.2196	0.2121
B	25.00	0.1718	0.1867	0.1728
C	8.33	0.1606	0.1489	0.1605
D	2.78	0.07765	0.071	0.0822
E	0.93	0.03135	0.0286	0.03775
F	0.31	0.01175	0.00955	0.0126
G	0.10	0.00485	0.00375	0.00415
H	0.03	0	0.00025	0

Yield (mg/mL): 2.804 Dilution Factor: 100×

Protein purified on 8/30/2022 and assayed on 8/31/2022.
Data uploaded by mspiridonova and last updated on 2022-09-15 21:48:35.

Design Data

Rosetta Score for S169A: -2.197

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.