D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I244K

Expression Data

Yield (mg/mL): 3.476

Kinetic Assay Data

**slope of I244K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1100	0.1158	0.1260
B	25.00	0.08905	0.08600	0.08600
C	8.33	0.05145	0.05560	0.05455
D	2.78	0.02270	0.02150	0.01950
E	0.93	0.008850	0.008250	0.007850
F	0.31	0.003000	0.003000	0.002750
G	0.10	0.001200		0.001300
H	0.03	0.0003000	0.0004000	0.000120

Yield (mg/mL): 3.476 Dilution Factor: 100×

Protein purified on 8/29/2022 and assayed on 8/30/2022.
Data uploaded by cgyeow and last updated on 2022-09-13 12:48:34.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.6285	0.5793	0.5627
B	25.00	0.4523	0.4218	0.4231
C	8.33	0.3396	0.3309	0.3122
D	2.78	0.1889	0.2053	0.1883
E	0.93	0.07200	0.08010	0.07240
F	0.31	0.03060	0.03560	0.03265
G	0.10	0.009000	0.01010	0.01205
H	0.03	0.0002500	0.0002500	0.0002500

Yield (mg/mL): 4.238 Dilution Factor: 100×

Protein purified on 8/29/2022 and assayed on 8/30/2022.
Data uploaded by cgyeow and last updated on 2022-09-13 12:42:14.

Design Data

Rosetta Score for I244K: 4.344

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.