D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F243H

Expression Data

Yield (mg/mL): 0.716

Kinetic Assay Data

**slope of F243H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1331	0.1252	0.1241
B	25.00	0.06670	0.05480	0.05670
C	8.33	0.03985	0.04300	0.05290
D	2.78	0.01840	0.02530	0.02355
E	0.93	0.01240	0.01280	0.01065
F	0.31	0.004800	0.004950	0.004850
G	0.10	0.001750	0.001750	0.001850
H	0.03	0.0002500	0.0003000	0.0003000

Yield (mg/mL): 0.716 Dilution Factor: 100×

Protein purified on 8/29/2022 and assayed on 8/30/2022.
Data uploaded by cgyeow and last updated on 2022-09-13 12:45:42.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.6285	0.5793	0.5627
B	25.00	0.4523	0.4218	0.4231
C	8.33	0.3396	0.3309	0.3122
D	2.78	0.1889	0.2053	0.1883
E	0.93	0.07200	0.08010	0.07240
F	0.31	0.03060	0.03560	0.03265
G	0.10	0.009000	0.01010	0.01205
H	0.03	0.0002500	0.0002500	0.0002500

Yield (mg/mL): 4.238 Dilution Factor: 100×

Protein purified on 8/29/2022 and assayed on 8/30/2022.
Data uploaded by cgyeow and last updated on 2022-09-13 12:42:14.

Design Data

Rosetta Score for F243H: 1.197

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.