D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H379Y

Expression Data

Yield (mg/mL): 1.046

Kinetic Assay Data

**slope of H379Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.01055	0.01085	0.0106
B	25.00	0.00875	0.0079	0.0089
C	8.33	0.0065	0.00785	0.0065
D	2.78	0.0036		0.00405
E	0.93	0.00175	0.00185	0.00205
F	0.31	0.0008	0.00085	0.00095
G	0.10	0.00045	0.00045	0.00045
H	0.03	0	0	0.0002

Yield (mg/mL): 1.046 Dilution Factor: 100×

Protein purified on 8/30/2022 and assayed on 8/31/2022.
Data uploaded by mspiridonova and last updated on 2022-09-07 16:06:42.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2197	0.2196	0.2121
B	25.00	0.1718	0.1867	0.1728
C	8.33	0.1606	0.1489	0.1605
D	2.78	0.07765	0.071	0.0822
E	0.93	0.03135	0.0286	0.03775
F	0.31	0.01175	0.00955	0.0126
G	0.10	0.00485	0.00375	0.00415
H	0.03	0	0.00025	0

Yield (mg/mL): 2.804 Dilution Factor: 100×

Protein purified on 8/30/2022 and assayed on 8/31/2022.
Data uploaded by mspiridonova and last updated on 2022-09-15 21:48:35.

Design Data

Rosetta Score for H379Y: -1.059

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.