D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L108N

Expression Data

Yield (mg/mL): 2.91

Kinetic Assay Data

**slope of L108N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.74E-01	2.86E-01	2.79E-01
B	25.00	2.42E-01	2.35E-01	2.31E-01
C	8.33	1.82E-01	1.88E-01	1.62E-01
D	2.78	1.20E-01	1.80E-01	6.53E-02
E	0.93	3.52E-02	7.77E-02	5.05E-02
F	0.31	2.20E-02	2.21E-02	2.00E-02
G	0.10	9.05E-03	9.10E-03	7.95E-03
H	0.03	0	3.00E-04	2.50E-04

Yield (mg/mL): 2.91 Dilution Factor: 100×

Protein purified on 8/10/2022 and assayed on 8/10/2022.
Data uploaded by kasiapno and last updated on 2022-08-17 21:01:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1932	0.1752	0.1723
B	25.00	0.1592	0.1479	0.1173
C	8.33	0.1107	0.1063	0.1151
D	2.78	0.05785	0.06060	0.05295
E	0.93	0.01915	0.01815
F	0.31	0.007200	0.005800	0.004400
G	0.10	0.003200	0.003000
H	0.03	0	0.0001500	0.0002000

Yield (mg/mL): 0.298 Dilution Factor: 10×

Protein purified on 8/10/2022 and assayed on 8/10/2022.
Data uploaded by kasiapno and last updated on 2022-08-15 17:38:09.

Design Data

Rosetta Score for L108N: -0.015

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.