Raw Data for BglB Variant Q313A

Expression Data

Yield (mg/mL): 1.254

Kinetic Assay Data

slope of Q313A A_{420 nm} (10⁻³ min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	166.2	226.4	209.9
	B	25.00	209.1	204.1	193.9
	C	8.33	153.2	149.1	148.5
	D	2.78	81.6	90.8	81.7
	E	0.93		24.9	27.4
	F	0.31	9.6	9.8	10.3
	G	0.10	3.5	3.8	3.6
	H	0.03	0	0.2	0.2

Yield (mg/mL): 1.254     Dilution Factor:  100×

Download these data

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by hseitz and last updated on 2022-07-28 21:29:56.

Reference Data for WT

slope of WT A_{420 nm} (10⁻³ min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	367.3	390.7	398.6
	B	25.00	328.7	325.6	327.9
	C	8.33	240.6	233	234.9
	D	2.78	139.3	136.3	132.9
	E	0.93	57	59.1	58.2
	F	0.31	21.3	22.1	21.8
	G	0.10	8.2	7.5	7.4
	H	0.03	0	0.2	1.8

Yield (mg/mL): 3.264     Dilution Factor:  100×

Download these data

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by hseitz and last updated on 2022-07-28 20:57:22.

Thermostability Assay Data

slope of Q313A A_{420 nm} (10⁻³ min⁻¹)

		T (°C)	1	2	3
	A	45.0	1.1	1.4	1
	B	44.2	2.6	2.4	2.1
	C	42.9	17.9	18.5	20.8
	D	41.3	198.3	223	220.7
	E	38.9	694.1	663.3	624.6
	F	36.8	946	935.5	910.3
	G	35.6	987.8	1016.3	951.2
	H	35.0	1090.8	1001	1041.6

Download these data

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by hseitz and last updated on 2022-07-28 21:31:49.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Citations

The unpublished data above were collected by students from Johnson County Community College. For inquiries, please contact Heather Seitz: <hseitz@jccc.edu>.