D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q313L

Expression Data

Yield (mg/mL): 3.9

Kinetic Assay Data

**slope of Q313L A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	408.7	423.3	430.2
B	25.00	339.7	351.3	343.3
C	8.33	285.6	299.1	292.8
D	2.78	186.7	190.6	184.3
E	0.93	90.3	96.2	93.4
F	0.31		35.7	34.8
G	0.10	12.7	12.6	14.2
H	0.03	0.1	0.3	0.2

Yield (mg/mL): 3.9 Dilution Factor: 100×

Protein purified on 3/27/2022 and assayed on 7/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:17:03.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	331.6	311.7	310
B	25.00	248.6	224.1	240.2
C	8.33	202.1	219.5	193.2
D	2.78	157	139.3	159
E	0.93	88.6	78.7	67.9
F	0.31	37.6	31.5	27
G	0.10	13.8	12.8	11.5
H	0.03	0.2	0.3	0.4

Yield (mg/mL): 2.6 Dilution Factor: 100×

Protein purified on 3/27/2022 and assayed on 7/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:12:28.

Thermostability Assay Data

**slope of Q313L A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	45.0	0.002100	0.002500	0.002500
B	44.2	0.007700	0.008600	0.005900
C	42.9		0.05360	0.05530
D	41.3	0.3260	0.3406	0.2994
E	38.9	1.016	0.9041	1.222
F	36.8	1.112	1.246	1.243
G	35.6	1.199	1.364	1.386
H	35.0	1.432	1.425	1.284

Protein purified on 2/27/2022 and assayed on 2/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:17:41.

Reference Data for WT

**slope of Q313L A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	45.0	0.0052	0.0052	0.0051
B	44.2	0.0149	0.0158
C	42.9	0.0685	0.093	0.1045
D	41.3	0.3375	0.4125	0.4341
E	38.9	1.187	0.9029	1.093
F	36.8	1.098	1.063	1.02
G	35.6	1.305	1.291	1.231
H	35.0	1.277	1.371	1.282

Protein purified on 2/27/2022 and assayed on 2/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:13:00.

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.