Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (10⁻³ min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	331.6	311.7	310
	B	25.00	248.6	224.1	240.2
	C	8.33	202.1	219.5	193.2
	D	2.78	157	139.3	159
	E	0.93	88.6	78.7	67.9
	F	0.31	37.6	31.5	27
	G	0.10	13.8	12.8	11.5
	H	0.03	0.2	0.3	0.4

Yield (mg/mL): 2.6     Dilution Factor:  100×

Download these data

Protein purified on 3/27/2022 and assayed on 7/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:12:28.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	45.0	0.0052	0.0052	0.0051
	B	44.2	0.0149	0.0158
	C	42.9	0.0685	0.093	0.1045
	D	41.3	0.3375	0.4125	0.4341
	E	38.9	1.187	0.9029	1.093
	F	36.8	1.098	1.063	1.02
	G	35.6	1.305	1.291	1.231
	H	35.0	1.277	1.371	1.282

Download these data

Protein purified on 2/27/2022 and assayed on 2/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:13:00.

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.