D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A145E

Yield (mg/mL): 2.618

**slope of A145E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2141	0.2273	0.2311
B	25.00	0.1685	0.1860	0.1899
C	8.33	0.1318	0.1440	0.1404
D	2.78	0.08785	0.08795	0.08455
E	0.93	0.03900	0.04345	0.04220
F	0.31	0.01610	0.01795	0.01775
G	0.10	0.005950	0.007200	0.007350
H	0.03	0.002850	0.002950	0.003300

Yield (mg/mL): 2.618 Dilution Factor: 100×

Protein purified on 7/27/2022 and assayed on 7/28/2022.
Data uploaded by thomasa and last updated on 2022-07-28 21:02:57.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3500	0.3489	0.3449
B	25.00	0.2942	0.2738	0.2824
C	8.33	0.2351	0.2236	0.2178
D	2.78	0.1344	0.1513	0.1476
E	0.93	0.06125	0.06905	0.07145
F	0.31	0.02450	0.02915	0.02825
G	0.10	0.009350	0.01090	0.01130
H	0.03		0.004800	0.005000

Yield (mg/mL): 3.264 Dilution Factor: 100×

Protein purified on 7/27/2022 and assayed on 7/28/2022.
Data uploaded by thomasa and last updated on 2022-07-28 20:49:17.

**slope of A145E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	35.0
B	35.6
C	36.8	0.7892	0.7721	0.7782
D	38.9	0.5457	0.6362	0.6426
E	41.3	0.2140	0.2416	0.2670
F	42.9	0.05330	0.03540	0.04630
G	44.2	0.007600	0	0.007600
H	45.0	0.002900	0.003100	0.003000

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by thomasa and last updated on 2022-07-28 21:24:55.

**slope of A145E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	35.0
B	35.6
C	36.8	0.8227	0.5690	0.9194
D	38.9	0.9212	1.022	0.8387
E	41.3	0.4718	0.4307	0.4794
F	42.9	0.1115	0.1044
G	44.2	0.02490		0.02660
H	45.0	0.007900	0.008000	0

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by thomasa and last updated on 2022-07-28 21:36:06.

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.