D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220Y

Expression Data

Yield (mg/mL): 3.234

Kinetic Assay Data

**slope of N220Y A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	403.7	411.4	403.2
B	25.00	343.6	335.1	337.6
C	8.33	244.4	245.5	250.1
D	2.78	145.8	140.6	144.8
E	0.93	63.6	62.9	62.9
F	0.31	23	22.3	22.9
G	0.10	7.9	9	8.2
H	0.03	0.1		0.1

Yield (mg/mL): 3.234 Dilution Factor: 100×

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by hseitz and last updated on 2022-07-28 20:54:24.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	367.3	390.7	398.6
B	25.00	328.7	325.6	327.9
C	8.33	240.6	233	234.9
D	2.78	139.3	136.3	132.9
E	0.93	57	59.1	58.2
F	0.31	21.3	22.1	21.8
G	0.10	8.2	7.5	7.4
H	0.03	0	0.2	1.8

Yield (mg/mL): 3.264 Dilution Factor: 100×

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by hseitz and last updated on 2022-07-28 20:57:22.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Citations

The unpublished data above were collected by students from Johnson County Community College. For inquiries, please contact Heather Seitz: <hseitz@jccc.edu>.