Raw Data for Wild-Type BglB

Expression Data

SDS-PAGE
 3.264

Kinetic Assay Data

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3500 0.3489 0.3449
B 25.00 0.2942 0.2738 0.2824
C 8.33 0.2351 0.2236 0.2178
D 2.78 0.1344 0.1513 0.1476
E 0.93 0.06125 0.06905 0.07145
F 0.31 0.02450 0.02915 0.02825
G 0.10 0.009350 0.01090 0.01130
H 0.03 0.004800 0.005000
 3.264      100×

Protein purified on 7/27/2022 and assayed on 7/28/2022.
Data uploaded by thomasa and last updated on 2022-07-28 20:49:17.



Thermostability Assay Data

slope of WT A420 nm (min−1)
T (°C) 1 2 3
A 35.0
B 35.6
C 36.8 0.8227 0.5690 0.9194
D 38.9 0.9212 1.022 0.8387
E 41.3 0.4718 0.4307 0.4794
F 42.9 0.1115 0.1044
G 44.2 0.02490 0.02660
H 45.0 0.007900 0.008000 0

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by thomasa and last updated on 2022-07-28 21:36:06.



Citations

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.