Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.102	0.092
	B	25.00	0.0822	0.0765
	C	8.33	0.0718	0.0765
	D	2.78	0.0406	0.0412
	E	0.93	0.0163	0.0197
	F	0.31	0.0086	0.0076
	G	0.10	0.0028	0.0034
	H	0.03	0.001	0.0009

Yield (mg/mL): 0.83     Dilution Factor:  100×

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Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by grahno02 and last updated on 2022-07-28 16:16:53.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	3.80E-01
	B	31.8	4.37E-01
	C	33.6	3.47E-01
	D	35.5	0.3655
	E	37.3	0.3835
	F	39.1	0.3743
	G	40.9	0.22
	H	42.7	0.0204
	I	44.5	0.0008
	J	46.4	0.0003
	K	48.1	0
	L	50.0	0

Download these data

Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by grahno02 and last updated on 2022-07-28 16:17:05.

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.