D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E388G

Expression Data

Yield (mg/mL): 5.114

Kinetic Assay Data

**slope of E388G A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2193	0.2280	0.2635
B	25.00	0.2823	0.2700	0.2602
C	8.33	0.2314	0.2390	0.2399
D	2.78	0.1794	0.1875	0.1835
E	0.93	0.08920	0.08341	0.08010
F	0.31	0.03375	0.03030	0.03020
G	0.10	0.01190	0.01190	0.01295
H	0.03		0.0002500	0.0002500

Yield (mg/mL): 5.114 Dilution Factor: 100×

Protein purified on 7/20/2022 and assayed on 7/20/2022.
Data uploaded by Nana-Awawu and last updated on 2022-07-26 18:27:14.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2450	0.2404	0.2372
B	25.00	0.2153	0.2000	0.2054
C	8.33	0.1507	0.1449	0.1483
D	2.78	0.09315	0.1166	0.1011
E	0.93	0.05545	0.06975	0.05970
F	0.31	0.02245		0.02270
G	0.10	0.008750		0.008600
H	0.03	0	0	0.0002500

Yield (mg/mL): 2.842 Dilution Factor: 100×

Protein purified on 7/20/2022 and assayed on 7/20/2022.
Data uploaded by Nana-Awawu and last updated on 2022-07-28 21:04:02.

Design Data

Rosetta Score for E388G: -4.416

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.