D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y166H

Expression Data

Yield (mg/mL): 1.282

Kinetic Assay Data

**slope of Y166H A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	170.1	160.7	175.2
B	25.00	129.4	131	138.6
C	8.33	72.8	62.5	80.4
D	2.78	29.4	29.2	38
E	0.93	11.5	12.4	11.7
F	0.31	5.3	3.7	8
G	0.10	1.2	1.3	1.5
H	0.03	0.7	0.5	0.8

Yield (mg/mL): 1.282 Dilution Factor: 100×

Protein purified on 7/18/2022 and assayed on 7/19/2022.
Data uploaded by morgan55 and last updated on 2022-07-20 16:12:47.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	306	309	275.7
B	25.00	256.3	240	275.4
C	8.33	230.8	211.8	231.2
D	2.78	105.7	115.3	128.6
E	0.93	43.8	44.3	47.1
F	0.31	16.1	14.7	17.3
G	0.10	5.5	5	6.3
H	0.03	0.7	0	0.3

Yield (mg/mL): 2.872 Dilution Factor: 100×

Protein purified on 7/18/2022 and assayed on 7/19/2022.
Data uploaded by morgan55 and last updated on 2022-07-20 14:35:54.

Design Data

Rosetta Score for Y166H: 3.44

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.