D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant M221K

Expression Data

Yield (mg/mL): 0.238

Kinetic Assay Data

**slope of M221K A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	65.8	70.3	62.8
B	25.00	53.5	54.4	50.9
C	8.33	43.2	40.3	34.5
D	2.78	21.8	17.6	17.6
E	0.93	7.6	7.5	7.1
F	0.31	2.9	2.9	2.6
G	0.10	1.2	1.4	1.2
H	0.03	0.3	0	0.5

Yield (mg/mL): 0.238 Dilution Factor: 10×

Protein purified on 7/18/2022 and assayed on 7/19/2022.
Data uploaded by morgan55 and last updated on 2022-07-20 14:45:50.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	306	309	275.7
B	25.00	256.3	240	275.4
C	8.33	230.8	211.8	231.2
D	2.78	105.7	115.3	128.6
E	0.93	43.8	44.3	47.1
F	0.31	16.1	14.7	17.3
G	0.10	5.5	5	6.3
H	0.03	0.7	0	0.3

Yield (mg/mL): 2.872 Dilution Factor: 100×

Protein purified on 7/18/2022 and assayed on 7/19/2022.
Data uploaded by morgan55 and last updated on 2022-07-20 14:35:54.

Design Data

Rosetta Score for M221K: 5.526

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.