D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K68H

Expression Data

Yield (mg/mL): 2.785

Kinetic Assay Data

**slope of K68H A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.36E+02	3.25E+02	3.34E+02
B	25.00	2.52E+02	2.71E+02	2.54E+02
C	8.33	2.24E+02	2.52E+02	2.09E+02
D	2.78	1.51E+02	1.29E+02	1.34E+02
E	0.93	6.00E+01	8.08E+01	7.91E+01
F	0.31	2.26E+01	2.45E+01	2.11E+01
G	0.10	7.90E+00	1.01E+01	7.20E+00
H	0.03	5.00E-01	1.00E-01	2.00E-01

Yield (mg/mL): 2.785 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by Sarahbob and last updated on 2022-07-15 13:49:30.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.99E+02	3.14E+02	3.10E+02
B	25.00	2.48E+02	2.45E+02	2.42E+02
C	8.33	2.31E+02	1.97E+02	2.11E+02
D	2.78	1.27E+02	1.27E+02	1.16E+02
E	0.93	4.03E+01	5.35E+01	5.93E+01
F	0.31	1.33E+01	2.26E+01	1.68E+01
G	0.10	6.10E+00	6.70E+00	5.70E+00
H	0.03		2.00E-01	2.00E-01

Yield (mg/mL): 2.833 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by Sarahbob and last updated on 2022-07-15 13:31:38.

Design Data

Rosetta Score for K68H: 2.387

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.