D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K413Q

Expression Data

Yield (mg/mL): 3.015

Kinetic Assay Data

**slope of K413Q A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	354.3	358.1	317.9
B	25.00	258.2	272	245.3
C	8.33	184	198.5	222.6
D	2.78	141	140.5	125.7
E	0.93	50.8	51.5	53.2
F	0.31	17.6	24.2	19.3
G	0.10	5.3	7.9	6.5
H	0.03	0.2	0.1	0

Yield (mg/mL): 3.015 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by dyoustra and last updated on 2022-07-15 13:36:46.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	60.3	6.53E+01	6.33E+01
B	25.00	45.8	4.56E+01	4.83E+01
C	8.33	4.47E+01	4.57E+01	3.83E+01
D	2.78	2.53E+01	2.81E+01	2.25E+01
E	0.93	1.18E+01	1.31E+01	9.10E+00
F	0.31	4.50E+00	4.50E+00	3.20E+00
G	0.10	1.90E+00	1.90E+00
H	0.03		1.00E-01	1.00E-01

Yield (mg/mL): 0.707 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by dyoustra and last updated on 2022-07-15 14:52:56.

Design Data

Rosetta Score for K413Q: 5.01

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.