D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L108M

Expression Data

Yield (mg/mL): 2.347

Kinetic Assay Data

**slope of L108M A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.69E+02	2.73E+02	2.77E+02
B	25.00	2.11E+02	2.21E+02	2.16E+02
C	8.33	1.80E+02	1.82E+02	1.77E+02
D	2.78	1.06E+02	1.09E+02	1.19E+02
E	0.93	6.84E+01	5.13E+01	5.29E+01
F	0.31	2.55E+01	2.40E+01
G	0.10	1.14E+01	5.40E+00	8.00E+00
H	0.03	3.60E+00		9.00E-01

Yield (mg/mL): 2.347 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by Sarahbob and last updated on 2022-07-15 13:39:11.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.99E+02	3.14E+02	3.10E+02
B	25.00	2.48E+02	2.45E+02	2.42E+02
C	8.33	2.31E+02	1.97E+02	2.11E+02
D	2.78	1.27E+02	1.27E+02	1.16E+02
E	0.93	4.03E+01	5.35E+01	5.93E+01
F	0.31	1.33E+01	2.26E+01	1.68E+01
G	0.10	6.10E+00	6.70E+00	5.70E+00
H	0.03		2.00E-01	2.00E-01

Yield (mg/mL): 2.833 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by Sarahbob and last updated on 2022-07-15 13:31:38.

Design Data

Rosetta Score for L108M: 0.629

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.