D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409R

Expression Data

Yield (mg/mL): 1.953

Kinetic Assay Data

**slope of W409R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.98E-02	1.11E-01	1.13E-01
B	25.00	1.08E-01	1.17E-01	9.79E-02
C	8.33	8.25E-02	7.43E-02	7.19E-02
D	2.78	5.82E-02	6.22E-02	5.73E-02
E	0.93	2.92E-02	2.87E-02	3.07E-02
F	0.31	1.37E-02	1.28E-02	1.56E-02
G	0.10	5.50E-03	4.75E-03	4.20E-03
H	0.03		2.50E-04	2.50E-04

Yield (mg/mL): 1.953 Dilution Factor: 100×

Protein purified on 5/11/22 and assayed on 5/11/22.
Data uploaded by wateraus and last updated on 2022-06-30 17:45:27.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.07E-01	8.71E-01	8.76E-01
B	25.00	7.51E-01	8.07E-01	8.13E-01
C	8.33	5.88E-01	6.61E-01	6.45E-01
D	2.78	4.07E-01	3.27E-01	3.94E-01
E	0.93	1.56E-01	1.23E-01	1.80E-01
F	0.31	6.61E-02	4.79E-02	7.30E-02
G	0.10	2.47E-02		2.52E-02
H	0.03	8.50E-04	6.00E-04	7.50E-04

Yield (mg/mL): 3.557 Dilution Factor: 100×

Protein purified on and assayed on .
Data uploaded by clairehuntington and last updated on 2023-11-07 18:27:52.

Design Data

Rosetta Score for W409R: 4.761

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.