D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404M

Expression Data

Yield (mg/mL): 1.866

Kinetic Assay Data

**slope of N404M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3362	0.3453	0.3574
B	25.00	0.2453	0.2480	0.2367
C	8.33	0.1476	0.1595	0.1514
D	2.78	0.08826	0.09936	0.09313
E	0.93	0.06007	0.06394	0.06075
F	0.31	0.05074	0.05270	0.05009
G	0.10	0.04799	0.04768	0.04534
H	0.03		0.04290	0.04302

Yield (mg/mL): 2.385 Dilution Factor: 10×

Protein purified on 4/25/2022 and assayed on 4/26/2022.
Data uploaded by cgyeow and last updated on 2022-06-27 07:10:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4766	0.4738	0.4752
B	25.00	0.3862	0.3683	0.3779
C	8.33	0.2816	0.2799	0.2808
D	2.78	0.1566	0.1498	0.1532
E	0.93	0.06110	0.06675	0.06396
F	0.31	0.02245	0.02340	0.02293
G	0.10	0.01025	0.007901	0.009076
H	0.03		0.0002500	0.0002500

Yield (mg/mL): 3.746 Dilution Factor: 100×

Protein purified on 6/2/2022 and assayed on 6/3/2022.
Data uploaded by cgyeow and last updated on 2022-06-27 07:04:03.

Design Data

Rosetta Score for N404M: -3.406

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.