D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant I299R

Expression Data

Yield (mg/mL): 4.558

Kinetic Assay Data

**slope of I299R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3494	0.3866	0.3615
B	25.00	0.2910	0.3189	0.2963
C	8.33	0.1991	0.2029	0.2178
D	2.78	0.1755	0.2073	0.2154
E	0.93	0.06595	0.06995	0.08100
F	0.31	0.02730	0.02535	0.02760
G	0.10	0.008150	0.008300	0.008150
H	0.03	0.0003000	0.0002500	0.0002500

Yield (mg/mL): 4.558 Dilution Factor: 100×

Protein purified on 6/1/2022 and assayed on 6/2/2022.
Data uploaded by cgyeow and last updated on 2022-06-27 07:10:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4766	0.4738	0.4752
B	25.00	0.3862	0.3683	0.3779
C	8.33	0.2816	0.2799	0.2808
D	2.78	0.1566	0.1498	0.1532
E	0.93	0.06110	0.06675	0.06396
F	0.31	0.02245	0.02340	0.02293
G	0.10	0.01025	0.007901	0.009076
H	0.03		0.0002500	0.0002500

Yield (mg/mL): 3.746 Dilution Factor: 100×

Protein purified on 6/2/2022 and assayed on 6/3/2022.
Data uploaded by cgyeow and last updated on 2022-06-27 07:04:03.

Design Data

Rosetta Score for I299R: 3.186

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.