Raw Data for BglB Variant C167S

Expression Data

Yield (mg/mL): 5.132

Kinetic Assay Data

slope of C167S A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.01345	0.01345	0.01310
	B	25.00	0.006100	0.006350	0.006050
	C	8.33	0.003600	0.003200	0.003150
	D	2.78	0.001300	0.001250	0.001250
	E	0.93	0.0006500	0.0007000	0.0006500
	F	0.31	0.0004000	0.0005500
	G	0.10	0.0003500	0.0004000
	H	0.03	0.0002500	0.0002000	0.0003000

Yield (mg/mL): 5.132     Dilution Factor:  100×

Download these data

Protein purified on 4/20/22 and assayed on 4/21/22.
Data uploaded by eandrejr and last updated on 2022-06-27 07:04:03.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.2586	0.2452	0.2764
	B	25.00	0.2196	0.2239	0.2194
	C	8.33	0.1850	0.1716	0.1921
	D	2.78	0.1039	0.1057	0.1200
	E	0.93	0.07245	0.07070	0.06070
	F	0.31	0.03155	0.03105	0.03710
	G	0.10	0.01520	0.01295	0.01200
	H	0.03	0.0003000	0.0001500	0.0003500

Yield (mg/mL): 3.809     Dilution Factor:  100×

Download these data

Protein purified on 4/19/22 and assayed on 4/20/22.
Data uploaded by eandrejr and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.