Raw Data for BglB Variant E177D

Expression Data

Yield (mg/mL): 1.93

Thermostability Assay Data

slope of E177D A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0006		0.0006
	B	48.3	0.0008	0.0008	0.0009
	C	45.7	0.0015	0.0014	0.0011
	D	42.4	0.0445	0.0521	0.0602
	E	37.7	0.4338	0.4775	0.4718
	F	33.6	0.5458	0.5733	0.5328
	G	31.3	0.585	0.6248	0.6197
	H	30.0	0.6673	0.6759	0.65

Download these data

Protein purified on 11/18/2021 and assayed on 11/28/2021.
Data uploaded by kieu123 and last updated on 2022-06-27 07:10:33.

Reference Data for WT

slope of E177D A_{420 nm}

		T (°C)	1	2	3
	A	50.0	0.0007000	0.0006000	0.0006000
	B	48.0	0.0008000	0.0006000	0.0009000
	C	46.0	0.001400	0.0009000	0.001100
	D	42.0	0.02240	0.01560	0.03040
	E	38.0	0.1276	0.1165	0.1060
	F	34.0	0.1500	0.1544	0.1416
	G	31.0	0.1791	0.1371	0.1639
	H	30.0	0.1869		0.1771

Download these data

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by nikchupkin and last updated on 2022-06-27 07:10:33.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.