D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant P251S

Expression Data

Yield (mg/mL): 3.591

Kinetic Assay Data

**slope of P251S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.09E-01	2.18E-01	2.17E-01
B	25.00	1.78E-01	1.80E-01	1.82E-01
C	8.33	1.68E-01	1.84E-01	1.67E-01
D	2.78	1.11E-01	9.03E-02	9.75E-02
E	0.93	3.69E-02	4.08E-02	4.07E-02
F	0.31	1.50E-02	1.74E-02	1.50E-02
G	0.10	5.40E-03	5.85E-03	5.40E-03
H	0.03	0	0

Yield (mg/mL): 3.591 Dilution Factor: 100×

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by pmanu002 and last updated on 2024-11-22 13:25:02.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.07E-01	3.02E-01	2.72E-01
B	25.00	2.52E-01	2.48E-01	2.46E-01
C	8.33	2.34E-01	2.43E-01	2.25E-01
D	2.78	1.20E-01	1.32E-01	1.35E-01
E	0.93	5.48E-02	5.48E-02	5.48E-02
F	0.31	2.07E-02	2.12E-02	2.22E-02
G	0.10	7.50E-03	7.05E-03	7.35E-03
H	0.03	0	7.50E-04	4.50E-04

Yield (mg/mL): 4.121 Dilution Factor: 100×

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by pmanu002 and last updated on 2024-11-22 13:08:38.

Thermostability Assay Data

**slope of P251S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		6.00E-04	7.50E-04
B	48.3	6.00E-04		6.00E-04
C	45.7	7.50E-04	7.50E-04
D	42.4	1.20E-03	1.50E-03	7.50E-04
E	37.7	2.16E-02	2.12E-02	2.03E-02
F	33.6	7.76E-02	7.74E-02	7.70E-02
G	31.3	9.38E-02	8.39E-02	8.12E-02
H	30.0	6.99E-02	7.67E-02	5.81E-02

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by pmanu002 and last updated on 2024-11-22 13:35:34.

Reference Data for WT

**slope of P251S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04		4.50E-04
B	48.3		6.00E-04	6.00E-04
C	45.7	9.00E-04	9.00E-04	1.05E-03
D	42.4		1.61E-02	1.59E-02
E	37.7	1.27E-01	1.39E-01	1.29E-01
F	33.6	1.67E-01	1.46E-01	1.58E-01
G	31.3	1.74E-01	1.56E-01	1.51E-01
H	30.0	1.51E-01	1.41E-01

Protein purified on 11/1/2024 and assayed on 11/8/2024.
Data uploaded by pmanu002 and last updated on 2024-11-22 13:12:21.

Design Data

Rosetta Score for P251S: -6.084

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.