D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220A

Expression Data

Yield (mg/mL): 0.66

Kinetic Assay Data

**slope of N220A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		2.19E-01	2.15E-01
B	25.00
C	8.33	2.41E-01		1.98E-01
D	2.78
E	0.93
F	0.31	1.70E-01	1.80E-01	1.60E-01
G	0.10	5.61E-02	5.03E-02	4.04E-02
H	0.03	1.65E-02	2.01E-02	1.88E-02

Yield (mg/mL): 0.66 Dilution Factor: 100×

Protein purified on 5/24/23 and assayed on 5/24/23.
Data uploaded by juliajoycameron and last updated on 2023-05-31 13:42:44.

Thermostability Assay Data

**slope of N220A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	4.00E-04	3.00E-04	6.00E-04
B	48.3	4.00E-04	4.00E-04	5.00E-04
C	45.7	4.00E-04	3.00E-04	6.00E-04
D	42.4	6.00E-04	6.00E-04
E	37.7	9.00E-04	1.10E-03	1.30E-03
F	33.6	1.10E-03		1.00E-03
G	31.3	1.90E-03	2.20E-03	2.80E-03
H	30.0	3.30E-03	3.50E-03

Protein purified on 5/24/23 and assayed on 5/24/23.
Data uploaded by juliajoycameron and last updated on 2023-05-31 13:29:02.

Design Data

Rosetta Score for N220A: 1.723

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.