D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220P

Expression Data

Yield (mg/mL): 3.792

Kinetic Assay Data

**slope of N220P A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.29E-02	7.79E-02	8.15E-02
B	25.00	6.23E-02	6.21E-02	6.32E-02
C	8.33	3.87E-02	3.93E-02	4.10E-02
D	2.78	2.04E-02	1.98E-02	2.06E-02
E	0.93	9.15E-03	9.00E-03	9.60E-03
F	0.31	3.60E-03	3.60E-03	3.90E-03
G	0.10	1.95E-03	1.80E-03	1.80E-03
H	0.03	6.00E-04	0	0

Yield (mg/mL): 3.792 Dilution Factor: 100×

Protein purified on 21/10/2022 and assayed on 28/10/2022.
Data uploaded by mcorr128 and last updated on 2022-11-26 12:00:45.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.13E-01	1.10E-01	1.11E-01
B	25.00	9.09E-02	8.88E-02	9.15E-02
C	8.33	6.57E-02	6.83E-02	6.77E-02
D	2.78	3.86E-02	3.65E-02	3.95E-02
E	0.93	2.24E-02	2.40E-02	2.28E-02
F	0.31	1.02E-02	9.75E-03	1.02E-02
G	0.10	4.80E-03	4.20E-03	5.25E-03
H	0.03	6.00E-04		7.50E-04

Yield (mg/mL): 2.294 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:08:58.

Thermostability Assay Data

**slope of N220P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	6.00E-04
B	48.3		4.50E-04	1.20E-03
C	45.7	4.50E-04	6.00E-04	7.50E-04
D	42.4	9.00E-04	6.00E-04	7.50E-04
E	37.7	3.90E-03	3.60E-03	3.60E-03
F	33.6	1.34E-02	1.29E-02	1.47E-02
G	31.3	1.74E-02	1.82E-02	1.76E-02
H	30.0		1.76E-02	1.65E-02

Protein purified on 21/10/2022 and assayed on 28/10/2022.
Data uploaded by mcorr128 and last updated on 2024-08-08 14:48:49.

Reference Data for WT

**slope of N220P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.3	6.00E-04		4.50E-04
C	45.7	6.00E-04	1.05E-03	7.50E-04
D	42.4	6.60E-03	6.45E-03	5.85E-03
E	37.7	3.74E-02	3.84E-02	3.56E-02
F	33.6	4.25E-02	4.16E-02	4.13E-02
G	31.3	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:16:50.

Design Data

Rosetta Score for N220P: 53.272

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.