About D2D Cure

The Design2Data Program involves students in an investigation of the sequence-structure-function relationship of proteins. Using the lens of protein biochemistry, students expand their knowledge and develop skills by engaging in a research workflow representative of cutting-edge biotechnology training in high demand by employers.

A feature that makes D2D unique among other multi-institutional protein biochemistry CUREs is its connection to the protein modeling research community, RosettaCommons, which uses the student-generated data to improve functionally predictive enzyme-design algorithms.

Meet Our Enzymes

β-glucosidase B (BglB)

2020 –

Species: Paenibacillus polymyxa

EC Number: 3.2.1.21
UniProt Number: P22505
PDB Entries: 2JIE 2O9P 2O9R 2O9T 2Z1S

Molar Mass: 51,573 Da
Extinction Coefficient (ε_BglB): 113,330 m⁻¹ cm⁻¹

View sequence

β-glucosidase B (BglB, lovingly called “Bagel B” by our teams) is an enzyme that catalyzes the hydrolysis of glucose monosacharides from larger molecules at a β-glycosidic linkage. It is an essential enzyme for the degradation of cellulose by bacteria and fungi.

View the pathway and mechanism.

Our computational designs and kinetic measurements will utilize para-nitrophenyl-β-d-glucopyranose (pNPG) as a colorimetric reporter substrate.

View the assay chemistry.

Next Enzyme

Our next enzyme system is coming soon.

Stay tuned!